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Image Search Results
Journal: Cell
Article Title: T-Scan: A Genome-wide Method for the Systematic Discovery of T Cell Epitopes
doi: 10.1016/j.cell.2019.07.009
Figure Lengend Snippet: (A) Overview of CMV screens. Peptides tiling across the CMV genome in 56-aa steps with 28-aa overlap are expressed via lentiviral transduction of target cells expressing HLA-A2, IFPGZB, and ICADCR. For NLV2 TCR T cells, donor CD8 T cells are transduced with lentivirus expressing the NLV2 TCR. For NLV-expanded T cells, CD8 T cells from a CMV-positive donor are expanded in the presence of the NLV peptide.
Article Snippet: CD8 T cells were purified from donor blood using the
Techniques: Transduction, Expressing
Journal: Cell
Article Title: T-Scan: A Genome-wide Method for the Systematic Discovery of T Cell Epitopes
doi: 10.1016/j.cell.2019.07.009
Figure Lengend Snippet: (A) Generation of memory CD8 T cells. Bulk memory CD8 T cells are purified from donor blood and undergo polyclonal expansion in the presence of feeder cells and anti-CD3.
Article Snippet: CD8 T cells were purified from donor blood using the
Techniques: Purification
Journal: Cell
Article Title: T-Scan: A Genome-wide Method for the Systematic Discovery of T Cell Epitopes
doi: 10.1016/j.cell.2019.07.009
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: CD8 T cells were purified from donor blood using the
Techniques: Recombinant, DNA Purification, Mutagenesis, Plasmid Preparation, Software